For wavelengths in the UV range above 220 nm, UV-clear plastic plates or quartz plates should be used. The measured bioluminescence data were expressed as mean ± SE (n = 3) and fitted with one-site binding model using the. For the Evans blue (EB) dye extravasation test, 3,8. Also refer to this Multimode Reader Comparison Chart. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. In a typical experiment 2 μL of freshly prepared 5-nitrobenzisoxazole stock solution in acetonitrile was added to 200 mL of buffered (10–25. 2013): the specific activity of both enzymes was ~1. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. A barcode reader accessory can be purchased with theIf you want to get the most sensitivity, you should set the plate reader excitation and emission wavelengths to different values for the two dyes. Bradford assays were conducted in 96-well plate format and absorption values recorded on a Molecular Devices SpectraMax M5 plate reader (Sunnyvale, CA). Reader Documentation Version No. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The decrease in absorbance at 340 nm was analyzed for the 200 s of the reaction, which corresponds to the initial rate of the reaction. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. edu to schedule training. 3. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:FlexStation® 3 Multi-Mode Microplate Reader. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. M5 plate reader at 595 nm. Most papers they cultured bacteria to OD600>0. 2. PPi. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. The fluorescence intensity was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif) with excitation/emission at 485/530 nm. Abs). Wide range of applications. Protocol for Qubit DNA BR Assay. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. All values reported represent the mean of at least three independent experiments. plates can be used in the SPECTRAmax PLUS 384. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. View the Molecular Devices website. Download : Download full-size image; Fig. Fe standards require only about 30 minutes but can be kept in 1x working solution for at least a week withoutAfter 15 min, the absorbance at 550 nm was recorded using a Molecular Devices SpectraMax M5 plate reader. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. PDE-lux transcriptional fusion plasmids were constructed as previously described. 2. Signals of wells containing only the target cells. Fluorescence was measured with the SpectraMax M5 plate reader with the same excitation and emission wavelengths as the chemical denaturation assay. CD spectra were recorded using a Jasco-715 circular dichroism spectrophotometer at 20 °C. Documents. 320 nm and em = 456 nm) on a Spectramax M5 plate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 9. PerkinElmer Envision plate readers with 2 reagent injectors; Kalypsys washer/dispenser system for rapid and precise dispensing and plate washing in 96, 384, 1536 formats. Guidelines to using the Qubit Flourimeter. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. The guide also covers the software features, the specifications, and the accessories of the systems. Help the planet as you cruise along at 5X speed. com GeneBLAzer® Assay Setup Guide on theThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The Back Panel Figure 1. Copia stocks a full line of used microplate readers. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. Models for In. 1. 5. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 2M posts 124. Thus, the. Most papers they cultured bacteria to OD600>0. Wells with an OD 600[0. Add 100 μL of this. Luminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET. One unit of hexokinase is the amount of enzyme that will generate 1. Fluorescence was recorded in a Spectramax M5 plate reader at a 0. Then, cells were treated with TCS of different concentra-tions (10, 20 and 40 μg/mL), according to theThe luminescent signal was quantified in a SpectraMax M5 plate reader (Molecular Devices, San Jose, CA). IC 50 values were determined on a multimode SpectraMax M5 plate reader using 96-well plates (Thermo Fisher Scientific) by monitoring absorbance at 412 nm taking measurements every 30 s for 20 min. During the room temperature incubation (step 9. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. Description. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. After 24 h, cells were treated as described for the individual figures, then assays were performed 48 h after transfection. 5. 3465 or email us. Information on how to reserve the plate readerMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Five. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. 2. [4] The IC 50 value of each drug was calculated by the Logit approach. 1. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. S. AK detection reagent (Lonza ToxiLight, 100 μL) was added to each well and the plate was incubated in the dark for 30 min at room temperature. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. was measured atdetermined450 nm using SpectraMax M5 plate reader. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. coli cells were purchased from New England Biolabs (Ipswitch, MA). 18632/oncotarget. Absorbance readings were then taken at 650 nm using a Spectramax M5 plate reader with SoftMax Pro v5. Table of Contents. Cultures were grown at 30°C and 900 rpm for 24 h, then OD600 was measured on a SpectraMax M5 plate reader to determine bacteriostatic potential. The WT DENV2C and its single-point mutants L50S, L54S, L81N and I88N were. begin reading microplates with seven mouse clicks. 5" H (50. 9000-0756. Bacterial growth was carried out at 25 °C in a 200 l volume withconstantshakingat450rpm. 9. NanoLuc substrate and R3/I5-SmBiT were added to the homogenate of HEK293T cells transiently overexpressing sLgBiT-fused receptor and bioluminescence was measured on a SpectraMax M5 plate reader with an interval of 30 s. Reader Documentation Version No. 2. Accordingly there are different configurations possible. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. Select Start>Control panel>Device Manager. 51 L/g). 9. Lucifer yellow was measured using an M5 plate reader (Molecular Probes) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only (blank) and four wells containing 50 nM ATP (sample). 7. RNA-Seq . For experiments with TGX-221, the cells were treated with 200 nM of TGX-221 throughout the duration of the experiment. Detection modes are available in UV-Vis absorbance, fluorescence, and. Fluorescence Microplate Reader Comparison Chart. All kinetic reads were taken at 25 °C in nonbinding all black clear-bottomed Greiner 96-well plates covered with optically clear films and stirred for 10 s prior to each reading. Description Figure 1. Open and turn on the laptop. Read More. The fluorescent intensity of the cells reflecting the LIP was quantified by measuring the fluorescent signal at excitation wavelength (λex) of 543 nm and emission wavelength (λem) of 580 nm as per the manufacturer recommendation using a SpectraMax M5 plate reader (Molecular Devices) and normalized to the cell count. 5. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). AC16 cells were treated with control, 5 mmol/L LiCl or 2. Optimal timing of signal detection occurred 15 min after reaction initiation with the kit substrates. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. Chemistry Department Facilities. Reactions were performed on a 384-well plate, with release of 6-FAM dye monitored by fluorescence at 517 nm using a SpectraMax M5 plate reader (Molecular Devices) with excitation at 495 nm for 60. Small molecule ligands that engage the regulatory. 25:1 effector to target cells, and the plates were incubated for an additional 3 hours. 0. Published Dec 16, 2020 | Updated Dec 16, 2020. Thesupplyofhumidifiedair (zero air) was controlled by a solenoid valve with on and offLastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. wavelength and 595 nm emission wavelength by Spectramax M5 plate reader (Molecular Devices). The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. Find regional support. The plate was incubated for 4 h and the absorbance of the solubilized formazan was measured on a Molecular Devices Spectramax M5 plate reader at 570 nm. Cell growth was monitored by measuring OD values at 600 nm using a SpectraMax M5 plate reader (Molecular Devices, Silicon Valley, CA, United States). SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 25% (v/v) AlamarBlue reagent was then added to cells and incubated in normal cell culture conditions for 1 h, protected from light. Cell Viability Assay Using Violet Staining The cells were seeded into 24-well plates and cultured in normal or growth medium or hormone-depleted medium with the relevant treatments as. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. Standards and QCs are composed of a. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The dual monochromator optics allow. Microplate Reader Shelf. Application Area: Multi mode plate reader. LbL films are immersed into 20 mM DTT in PBS buffer (2. 0 adjusted at rt containing 10% v/v DMSO) (100 μL) and a 2- or 5-fold dilution was. The purple holder is used for reading non-transparent plates from the top (e. Cell morphology HeLa & caski cells were, respectively, plated in 6-well plates to logarithmic growth phase. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. Microplate Reader, Multimode. Supernatants were collected, and the concentration of l-lactate was determined by measuring absorbance of reaction samples using the Spectra Max M5 plate reader (Molecular Devices). In the M2, M5 and Plus. 2 software for data reduction (Molecular Devices, Sunnyvale, CA). 95. Microplate Capacity. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Prepare a 1:1 solution of Greiss Reagent 1:Greiss Reagent 2. Molecular Devices M5 plate readers; Multiple liquid bulk dispensers (Wellmate, Multidrop Combi) Price List. The colorimetric reaction was stopped with 2 M sulfuric acid, and absorbance was measured at 450 nm using a SpectraMax M5 plate reader (Molecular Devices). About. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Choose the Experiments icon. Plate chamber design of the SpectraMax category 1 microplate readers. Product Overview. 4-liter V-8 that sends power to all four wheels via the car’s. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Cells stably expressing wild-type or E633K p110β were. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Viability was evaluated using the Cell Titer Glo Assay (Promega) and luminescence was read on a SpectraMax M5 plate reader (Molecular Devices). These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Table of Contents. Remove plate cover. The ultrapure water used in the experiments was prepared with a Millipore Direct-Q® 3 system (Merck Millipore, MA, U. For a Price List, please call (858) 646-3100 ext. To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. 5 pM (0. Absorbance measured on a Spectra Max M5 plate reader (Molecular Devices). The peptide concentration was. Luminescence was measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA) after the addition of nano-Glo substrate (Promega, Madison, WI) in accordance with the manufacturer’s directions. Tablet PC. For. Cell–cell fusion assays were also performed by transient transfection of pHXB2-env plasmid [34] along with pRev and pTat plasmids into 293T cells and these were added to the receptor. 2. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. Microplate Readers; SoftMax Pro Software; Microplate Stacker and Washer; Cellular Imaging Systems; Clone Screening; FLIPR; AXON Patch-Clamp; Assay Kits. 10. The BMW M5 CS is the end of an era, one final swan song for internal combustion M5s, and it sends its era out with a loud, violent, obnoxiously fast bang. 2. The software is simple to use and set up protocols. This procedure is illustrated in Fig. 5: Schematic of the back panel of a reader. Activity assays were performed in clear, flat-bottomed 96-well plates and the decrease in absorbance at 340 nm was recorded using a SpectraMax M5 plate reader (Molecular Devices). If the computer is connected to the Internet: Select Search Automatically for. between the affordability of filter-based readers and the . Fluorescence Microplate Readers. Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. The plate was washed and an anti-rabbit peroxidase-conjugated secondary antibody (Jackson Immunoresearch) was applied at a 1:3000 dilution in PBS. 5. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. SpectraTest ABS2 Absorbance Validation Package performance tests. 3. 0 and room temperature. Data reduction and calculation were performed with MSD Discovery workbench software (v 3. Wolfe1, and Stephen M. Fluorescence Microplate Reader Comparison Chart. 1 M NaCl at varying pH levels (3–10) and incubated at room temperature for 1 h. SpectraMax Readers have the most complete level of product validation and compliance. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 1. 40266 Email: [email protected] fluorescence intensity was determined using a fluorescent plate reader (SpectraMax M5 plate reader) set at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. plates with an 8-fold dispenser (Bio Tek Germany, Bad Friedrichshall, Germany) and the plates were then centrifuged for 1 min at 1,000 rpm and room temperature. White or black. The password is. Information on how to reserve the plate readerSunnyvale, California -- October 5, 2017. Duplicate wells were tested for each condition, and data were graphed as a percent of control in GraphPad Prism (GraphPad Software). 0112-0044C 3 Contents Chapter1:CuvettePortValidationOverview 4 CertificateofCalibration 5 ISO-17025 5 AvailableTests 6 Chapter2:UseValidationProtocols 7A SpectraMax M5 plate reader was able to continuously incubate the microtiter plate at 37°C and measure the fluorescence (λex/λex= 529/600 nm) every 5 minutes for 1. 1 software to identify the IC50. 4 software (Molecular Devices, San Jose, CA, USA). M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. #655801. PURPOSE. 1. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 17. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. To update the driver. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. 2% acetamide. The plate reader assay was then repeated in SC-URA media with and without. 3. The measured specific activity of NanoLuc is 1. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. 5. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. 2 mg/mL using 2 different UV-visible spectrophotometers, a SpectraMax M5 plate reader, and a NanoDrop instrument. 500 mm/s is the maximum speed of the extruder of AnkerMake M5 when using Fast Mode. 0 mM of NADH per min at pH 8. Discovery of CRBN as a target of thalidomide: a breakthrough for progress in the development of protein degraders. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 1, and the color is formed by the reaction in the microtiter plate. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. ). The EC50 values were calculated from the absorbance values vs. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: Step 4: Incubate at room temperature for 1 hour, protected from light. After mixing all the components, the absorbance of the mixture was continuously measured at 475 nm at 25°C using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). both cuvette-based and microplate reader applications on the same instrument. SpectraMax Injector Cartridge with SmartInject™ Technology. StakMax Handling Stacker Base Unit. Multimode Reader Comparison Chart. 5°C at 37°C. Top reading is usually more sensitive. Detection modes are available in UV-Vis absorbance, fluorescence, and. 2. Colorimetric detection of crystal violet released into the supernatant, which is proportional to the number of attached cells, was determined using a SpectraMax M5 plate reader (Molecular Devices). Select Update Driver. The antibiotic bacitracin A has been used as an inhibitor of thiol isomerases, including ERp5, in the insulin reductase assay . Thank you!. Luminescence was measured with an integration time of 1000 ms per well on a SpectraMax M5 plate. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. 1. Attune Flow Cytometer; FacsCalibur; ImageQuant 800; Leica Cryostat; M5 Plate Reader; Odyssey CLx Imager; Typhoon 5;Then, the cells were replaced for incubation for 60 min. Even better, they require MINIMAL modification to. 2. SpectraMax Injector Cartridge with SmartInject™ Technology. 11. xlsx file, with or without the averages and normalizations. The SpectraMax® ABS Plus Microplate Reader can run . After the plate is read, data will appear in the plate section: 11. Figure 9. The emission of roGFP2 was measured between 500 and 530 nm after excitation at 405 nm and 488 nm every 30 s in a SpectraMax M5 plate reader (Molecular Devices). The plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and. 7°C and the bottom to 36. Top reading is usually more sensitive. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 0 μM wild-type R3/I5. Copia stocks a full line of used microplate readers. After cell attachment, culture media was replaced with F-12 media containing 1% FBS for overnight incubation. T-test comparisons of group mean values (+/− SEM) were used to determine significance of genotype. pH-Dependent Solubility. Luminescence was detected using the Bright-Glo Luciferase Assay System (Promega) on a SpectraMax M5 plate reader (Molecular Devices). 1. AB-0558) and immediately read for luminescent signal on a SpectraMax M5 plate reader. A solution of phenol red-free MEM containing 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Fluorescence was measured using microplate reader at an excitation wavelength of 488 nm and emission wavelength of 529 nm using Spectramax-M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Fluorescence values were used to calculate the percent inhibition of enzyme activity relative to maximal activity, PTP4A3 in the absence of inhibitor, and maximal inhibition, PTP4A3 in the presence of 2 mM Na 3 VO 4. Viability Assay reagent (Promega). Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues are proactively addressed. 2. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. Select the Driver tab. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. Plate Analysis 9. Linear, Orbital Slow, and Orbital Fast. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. RoHS Compliant. In general, reading fluorescence from the top is more sensitive than reading from the bottom. The reduced readings obtained from the plate reader were analyzed using PRISM 6. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 2. The Formazan crystals formed in each well were dissolved by adding 100 µl DMSO. I’m. Web Information The compact FLx800™ fluorescence reader provides high performance in 6- to 384-well microplates at an attractive price. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. We therefore gathered salient. 2. 2. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. 1. Absorbance was measured at 563 nm using a SpectraMax M5 plate reader (Molecular Devices). You can use it for many applications (OD, fluorescence, luminescence). Circular Dichroism. Select Update Driver. 2000) supplemented with each chemical com-pound with the final concentration of 10 mmol l-1. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. 5 × 10 5 counts/fmol when measured with a Spectromax M5 plate reader using white opaque 96-well plates. Plate Reader with 5 modes of detection, UV-Vis (Abs), Fluorescence (FI) , Lumi, TRF, F Polarization, with current validations, fully inspectedOpen the Gen 5 software, 2. 5. Thank you!. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. Qualitative and quantitative data analysis, along with customizable exporting and reporting enable automated end-to. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. Simply unpack it, plug it in, and begin your experiments. For example, microplates containing different samp les can be read using the same orChoose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. com 9. -Glo assay solution (Promega) at room temperature and luminescence measured at 1000 ms −1 integration on a SpectroMax M5. 10569C 2 C 12 cells were treated exactly as described for free [Mg 2+] determination, except that MgG was replaced by 5 μM safranine O. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. SpectraMax M5 Plate Reader: 901F: Nanodrop 2001: BioRad ChemiDoc gel documentation (old) Bio-Rad T100Tm Thermal Cycler : Bio-Rad CFX ConnectTM Real-Time System : Location. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Protocol for Qubit DNA HS Assay. 2.